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In Vitro Test Results

L2 cells were exposed to 2.5% Essential GSH® and intracellular glutathione was measured after 30 minutes, 1 hour, 2 hours, 3 hours, and 4 hours. Intracellular glutathione content was measured via the spectrophotometric recycling assay, normalized to total cellular protein.

Results clearly indicate that intracellular glutathione levels increased after exposure to Essential GSH®, peaking at 2 hours with over a 2-fold increase. The transient action of the increased glutathione remains unknown, although repression of the endogenous glutamate-cysteine ligase (GCL) enzyme through GSH-feedback inhibition may be a likely reason.

Tests performed by:
Dale Dickinson, PhD
Assistant Professor
Department of Environmental Sciences
University of Alabama at Birmingham

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